Syphilis is a kind of global distribution of sexually transmitted disease, it almost affects all the organ system of human body, causing seriously damage to the patient's physical and mental health. When human infected Syphilis, it may produce a variety of antibodies, mainly composed of two specificanti leptospira antibodies of IgM and IgG. Another kind of nonspecific antibody callde reagin, is the antibody produced by the human body when stimulated by the lipid materials released by the histocyte damage by the spirochete, or spirochete itself. These can be test by serological experiment.